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F2426

EZview™ Red ANTI-FLAG® M2 Affinity Gel

clone M2

Synonym(s):

Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Application:
IP, affinity chromatography
Citations:
98
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clone

M2, monoclonal

Quality Level

200

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

matrix

4% agarose bead; 45-165μm bead size

isotype

IgG1

capacity

≥0.6 mg/mL, gel binding capacity

shipped in

wet ice

storage temp.

−20°C

General description

EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.

Application

Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.

Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

FLAG® Affinity Gels, FLAG® tag, 3xFLAG®tag, DYKDDDDK tag

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
048K6140
027K6073
124K6022
122K9166
105K6211

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

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Global Trade Item Number

SKUGTIN
F2426-1ML04061838255358
F2426-500UL04061838248640
F2426-5X1ML04061838255365