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Merck

XTG360-RO

Roche

X-tremeGENE™ 360 Transfection Reagent

Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines

Synonym(s):

transfection reagent

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About This Item

UNSPSC Code:
41106502
NACRES:
NA.55

Key Documents

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grade

Molecular Biology

form

liquid (aqueous solution)

packaging

pkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

−20°C

Related Categories

General description

X-tremeGENE™ 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE™ 360 delivers high efficiency transfection in more cell types with low cytotoxicity.

Application

X-tremeGENE™ 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.
  • Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
  • Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
  • Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
  • Suitable for transient and stable transfection.
  • Functions well in the presence or absence of serum.

Features and Benefits

X-tremeGENE™ 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE™ 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE™ 360 exhibits:
  • Peak performance in a broad range of cell types
  • Suitable for multiple applications
  • Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE™ 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Analysis Note

Each lot of X-tremeGENE™ 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

61.7 °F

Flash Point(C)

16.5 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
42905300
38804100
48374700
38802500
45274000

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Protocols

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

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