Skip to Content
Merck

1.03840

SAFC

Cellvento® 4CHO-X COMP

expansion medium

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
41106213

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

500

description

shipped with cooling packs

product line

Cellvento®

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

storage temp.

2-8°C

Related Categories

General description

There are several advantages to using perfusion in your seed train, namely, flexibility in your production processes, increase in capacity and footprint reduction. Reducing the number of bioreactors in your seed train leads to a better utilization and eliminates traditional bottlenecks (compressed seed train). Additionally, using N-1 perfusion enables the inoculation of a production bioreactor with higher cell densities for high seed production process formats. Cellvento® 4CHO-X Expansion Medium has been specifically developed for N-1 perfusion for Chinese Hamster Ovary (CHO) cell lines. It is formulated with a high nutrient concentration to achieve low cell specific perfusion rates (CSPRs) at high cell densities. The medium is chemically defined, of non-animal origin, and contains no hydrolysates or components of unknown composition.

Application

Cellvento® 4CHO-X Expansion Medium has been specifically formulated to control the metabolic profile of the cells, both to minimize toxic byproducts and to ensure adequate nutrient levels for high cell densities. Preventing nutrient depletion can increase robustness and predictability of seed train operations. This medium supports high growth and viability, thus increasing cell biomass for cryopreservation or inoculation of another reactor. The medium addresses seed train intensification and shows a positive impact in IgG production, when combined in perfusion production with EX-CELL® Advanced HD Perfusion medium (24370C), and in Fed-batch production with e.g. Cellvento® 4CHO medium (103795) and Cellvento® 4Feed (103796). It is expected to work with other media platforms as well. Cellvento® 4CHO-X Expansion Medium can be used for all seed train expansion steps including N-1, reaching robust growth at low CSPRs. This product is intended for research or further manufacturing but not for human or therapeutic use.

Preparation Note

Formulated to contain 26.2 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Cell Retention Technology and Specialized Cell Culture Media for Intensified Upstream Processing

How the Cellicon® filter and controller enables seamless adoption of a perfused N-1 seed train step into the cell expansion process.

CHO Media and Feeds for Optimized Production of Therapeutic Proteins

Cellvento® and EX-CELL® CHO cell culture media and feeds, optimized for monoclonal antibody & recombinant protein production in fed-batch & perfusion

Establishing the First GMP Guideline for Cell Culture Media

Cell culture media are vital for bioprocessing therapeutics. This page describes the central role of our organization in authoring purpose-built GMP for cell culture media.

Optimizing CHO Cells for Higher Productivity

Cellvento 4CHO-X Expansion Medium enhances cell biomass for bioreactor inoculation in cell expansion stages.

Related Content

Pengeluaran dan ketegangan Harvest

Pemaju biofarmaseutikal memerlukan proses huluan yang cekap dan produktif yang menyampaikan antibodi monoklonal berkualiti tinggi (MABS) dan lain-lain jenis protein rekombinan. Proses intensif boleh masa depan-bukti strategi pengeluaran mereka, mengurangkan kos, dan meningkatkan fleksibiliti kemudahan.

Benih Keretapi Intensification

Pemaju biofarmaseutikal memerlukan proses huluan yang cekap dan produktif yang menyampaikan antibodi monoklonal berkualiti tinggi (MABS) dan lain-lain jenis protein rekombinan. Proses intensif boleh masa depan-bukti strategi pengeluaran mereka, mengurangkan kos, dan meningkatkan fleksibiliti kemudahan.

Pembangunan Media budaya sel bagi MABS

Ketahui lebih lanjut tentang pembangunan media kultur sel untuk pembangunan dan pembuatan MAB serta produk yang anda perlukan untuk langkah ini.

Cell Culture Media and Feeds Selection Guide
See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service