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• PromoCell^® Brochure

  More physiologically relevant models using human primary cells.

• Primary Cell Culture FAQ

  Primary Cell Culture: Frequently Asked Questions (FAQs)

• Introduction Video to Primary Cell Culture

  Because they are derived directly from living tissue, primary cells maintain physiological relevance and thus find increasing use in life science research and pharmaceutical drug discovery. Compared to immortalized cell lines, however, primary cells can be finicky and present challenges during culture unless proper care is taken. Cell Applications recommends the user follow the steps outlined in these video segments to achieve optimal results with their primary cells.

• Primary Cell Culture Basics

  What are primary cells? A comprehensive overview of animal and human primary cell lines and media and how they are used in biomedical cell culture applications. Primary cultures are less homogeneous relative to cell lines.

• Generating organotypic 3D skin cultures as an in-vitro model of the human epidermis

  3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

• Primary Human Chondrocytes and Chondrocyte Growth Media

  Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

• Primary Human Fibroblasts and Growth Media

  Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

• Tumor Spheroid Formation Assay

  3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.

• Primary Human Endothelial Cells and Growth Media

  Primary human endothelial cells isolated from microvascular tissue and large blood vessels including HUVEC plus optimized growth media. Protocols for handling, cell passaging, media, and product use.

• Primary Human Mononuclear Cells and Growth Media

  Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

• Primary Airway Epithelial Cells and Growth Media

  Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.

• Primary Human Mononuclear Cells and Growth Media

  Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.