material
PE frit (20 μm)
polypropylene hardware
품질
MS friendly
구성
bed wt., 60 mg/well
포장
pk of 1
기술
solid phase extraction (SPE): suitable
표면적
160-420 m2/g
Matrix
Hydrophilic modified styrene polymer particle platform
기질 활성군
polystyrene phase
입자 크기
50-70 μm
공극 크기
80-200 Å pore size
응용 분야
food and beverages
분리 기술
reversed phase
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일반 설명
Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)
- Hydrophilic modified styrene polymer
- Developed for the extraction of a highly broad range of compounds from aqueous samples
- Retention predominately based on reversed-phase; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.
- Examples of more polar compounds that are retained and recovered include (but not limited to): pyridoxine (logPo/w -0.56), riboflavin (logPo/w -2.02), biotin (logPo/w 0.11).
특징 및 장점
- Excellent sample prep performance at a lower price
- Amenable to generic methodology - save time, money, and headache during method development
- Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
- Resistance to overdrying allows for more robust methdology
- Low UV and MS extractables for lower background and greater sensitivity
- Stringer production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision
법적 정보
Supel is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Chiedza F Munyeza et al.
Biomedical chromatography : BMC, 30(6), 837-845 (2015-09-18)
Tigecycline (TIG), a derivative of minocycline, is the first in the novel class of glycylcyclines and is currently indicated for the treatment of complicated skin structure and intra-abdominal infections. A selective, accurate and reversed-phase high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
Pablo Vazquez-Roig et al.
Analytical and bioanalytical chemistry, 397(7), 2851-2864 (2010-05-01)
A simple and robust method using solid-phase extraction (SPE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of 14 drugs of abuse and their metabolites (cocainics, amphetamine-like compounds, cannabinoids, and opiates) in surface waters has been developed.
Wei Chen et al.
Chemosphere, 163, 99-107 (2016-08-16)
A sensitive method for simultaneous determination of 20 trace organic chemicals (TOrCs, including preservatives, antioxidants, disinfectants, oestrogens, alkyl-phenols and bisphenol-A) in surface water and wastewater has been developed and validated based on the optimisation of solid-phase extraction (SPE) followed by
Martina Rejtharová et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 34(4), 477-481 (2016-12-06)
Monitoring of steroid esters in blood serum is desirable in order to detect the possible illegal use of natural hormones as growth promoters. A method for the determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate
문서
Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.
프로토콜
LC/MS Analysis of Opioid Glucuronide Metabolites in Urine on Ascentis® Express F5 after Solid Phase Extraction (SPE) using Supel™-Select HLB
Chromatograms
application for SPE, application for HPLC
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