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Merck

STR1

Sigma-Aldrich

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

동의어(들):

Reverse Transcriptase cDNA synthesis kit, eAMV-RT

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352200
NACRES:
NA.55

주요 문서

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기술 서비스
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도움 문의

Quality Level

200

사용

sufficient for 50 reactions
 kit sufficient for 50 reactions

특징

dNTPs included
hotstart: no

기술

RT-PCR: suitable

색상

colorless

입력

purified RNA

배송 상태

dry ice

저장 온도

−20°C

관련 카테고리

일반 설명

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

애플리케이션

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).

기타 정보

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

법적 정보

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

키트 구성품 전용

제품 번호
설명
  • Enhanced Avian Reverse Transcriptase 1000 U

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • Deoxynucleotide mix 50 μL
  • O4387Anchored oligo (dT)23 100 μLSDS
  • R7647Random nonamers 100 μLSDS
  • O4387Ribonuclease inhibitor 50 μLSDS
  • W1754PCR grade water 1.5 mLSDS

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCH8162
SLCH2897
SLCG8141
SLCG6019
SLCF4760

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

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모든 관련된 서류 보기

문서

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

프로토콜

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

관련 콘텐츠

PCR 애플리케이션

중합효소 연쇄 반응(PCR)은 핵산 분자를 증폭하는 기술로, RT-PCR, 핫 스타트 PCR, 엔드포인트 PCR 등 다양한 애플리케이션에서 일반적으로 사용됩니다.

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

RT-PCR Product Listing
RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

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