SRE0018
Lyticase from Arthrobacter Luteus
Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein
동의어(들):
Lyticase from Arthrobacter luteus
일반 설명
Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.
애플리케이션
For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).
생화학적/생리학적 작용
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
기타 정보
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Louis Dacquay et al.
G3 (Bethesda, Md.), 7(6), 1799-1809 (2017-04-30)
Actively proliferating cells constantly monitor and readjust their metabolic pathways to ensure the replenishment of phospholipids necessary for membrane biogenesis and intracellular trafficking. In
프로토콜
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.