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Merck

S5171

Sigma-Aldrich

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

동의어(들):

sFN

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

MDL number:
MFCD00284706
UNSPSC 코드:
12352202
NACRES:
NA.75

주요 문서

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기술 서비스
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제품 이름

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

생물학적 소스

human

Quality Level

200

재조합

expressed in E. coli

제품 라인

BioReagent

양식

solution

분자량

~235-270 kDa

포장

pkg of 5 mg

기술

cell culture | mammalian: suitable

표면 범위

1 μg/cm2

UniProt 수납 번호

P02751

배송 상태

ambient

저장 온도

2-8°C

유전자 정보

human ... FN1(2335)

관련 카테고리

애플리케이션

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

제조 메모

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

기타 정보

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

면책조항

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000115055
11180741
018M4061V
067M4133V
076M4047V

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

N Okada et al.
The Journal of biological chemistry, 272(43), 26978-26984 (1997-10-27)
The pathogenic Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) binds to fibronectin via protein F. In this study, we have investigated the binding properties of protein F to various multimeric tissue forms of fibronectin that appear on cell surfaces and
Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example
Giornale di Psichiatria e di Neuropatologia (2012)
Gentle cell handling with an ultra-slow instrument: creep-manipulation of cells
Thielecke H, et al.
Microscopica Acta, 11(11), 1230-1241 (2005)
Florian Weinberg et al.
International journal of molecular sciences, 21(23) (2020-12-03)
The epidermal growth factor receptor HER2 is overexpressed in 20% of breast cancer cases. HER2 is an orphan receptor that is activated ligand-independently by homodimerization. In addition, HER2 is able to heterodimerize with EGFR, HER3, and HER4. Heterodimerization has been
A Morla et al.
Nature, 367(6459), 193-196 (1994-01-13)
Fibronectin is an extracellular matrix protein that is important in development, wound healing and tumorigenesis. In the blood it is dimeric, but in tissues forms disulphide crosslinked fibrils. Here we show that a fragment from the first type-III repeat of

문서

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

프로토콜

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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