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Merck

P3910

Sigma-Aldrich

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

동의어(들):

Proteinase from Bacillus licheniformis, Subtilisin A

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
EC 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
eCl@ss:
32160410
NACRES:
NA.84
기술 서비스
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도움 문의

양식

lyophilized powder

Quality Level

품질

for use in Total Dietary Fiber Assay, TDF-100A

분자량

27 kDa

응용 분야

food and beverages

배송 상태

wet ice

저장 온도

2-8°C

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관련 카테고리

일반 설명

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

애플리케이션

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

생화학적/생리학적 작용

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

기타 정보

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
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The Journal of biological chemistry, 287(9), 6530-6538 (2012-01-12)
Quinohemoprotein amine dehydrogenase (QHNDH), an αβγ heterotrimer present in the periplasm of several Gram-negative bacteria, catalyzes the oxidative deamination of various aliphatic amines such as n-butylamine for assimilation as carbon and energy sources. The γ subunit of mature QHNDH contains

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