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Merck

M8823

Millipore

Anti-FLAG® M2 Magnetic Beads

affinity isolated antibody

동의어(들):

Monoclonal ANTI-FLAG® M2, FLAG® magnetic affinity resin, FLAG® resin for high throughput, Flag® Affinity resin, Anti-ddddk, Anti-dykddddk

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
NACRES:
NA.32

주요 문서

모든 문서 보기
기술 서비스
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도움 문의
기술 서비스
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도움 문의

결합

magnetic beads

Quality Level

200

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

M2, monoclonal

양식

suspension

유통기한

2 yr at -20 °C

분석물 화학적 분류

proteins

기술

affinity chromatography: suitable
immunoprecipitation (IP): suitable

비드(bead) 크기

20-75 μm

Matrix

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

동형

IgG1

용량

≥0.6 mg/mL binding capacity

배송 상태

wet ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

관련 카테고리

일반 설명

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

애플리케이션

Suitable for immunoprecipitation procedures.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Learn more product details in our FLAG® application portal.

특징 및 장점

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

물리적 형태

Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag

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당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCJ0317
SLCL3090
SLCJ1422
SLCK0459
SLCH4826

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Ephrem G Kassa et al.
PLoS pathogens, 15(6), e1007851-e1007851 (2019-06-27)
Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the apical plasma membrane of the small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, which
Chanqiong Zhang et al.
Cancer biology & therapy, 20(9), 1213-1222 (2019-04-16)
It is verified that long non-coding RNAs (lncRNAs) play crucial roles in various cancers. LncRNA LEF1-AS1 is a reported oncogene in colorectal cancer and glioblastoma. In this study, we unveiled that LEF1-AS1 markedly increased in oral squamous cell carcinoma (OSCC)
K Kollmann et al.
Leukemia, 31(4), 934-944 (2016-10-16)
Most myeloproliferative neoplasm (MPN) patients lacking JAK2 mutations harbour somatic CALR mutations that are thought to activate cytokine signalling although the mechanism is unclear. To identify kinases important for survival of CALR-mutant cells, we developed a novel strategy (KISMET) that
Lan-Lan Smith et al.
Cell stem cell, 8(6), 649-662 (2011-06-01)
Bmi1 is required for efficient self-renewal of hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs). In this study, we investigated whether leukemia-associated fusion proteins, which differ in their ability to activate Hox expression, could initiate leukemia in the absence
Eva Madi Riising et al.
PloS one, 3(7), e2704-e2704 (2008-07-17)
The Polycomb Repressive Complex 2 (PRC2) functions as a transcriptional repressor through a mechanism that involves methylation of Histone H3 at lysine 27. The PRC2 complex activity is essential for cellular proliferation, development, and cell fate decisions. PRC2 target genes
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문서

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

관련 콘텐츠

단백질 발현

단백질 발현 기술은 연구, 치료제, 백신 생산을 지원하는 다양한 발현 시스템에 사용됩니다.

단백질 정제

이온교환, 크기 배제 및 단백질 친화성 크로마토그래피를 포함한 방법을 사용하는 재조합형 단백질 정제를 위한 단백질 정제 기법, 시약 및 프로토콜.

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

모두 보기

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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