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Merck

L2500

Sigma-Aldrich

L-Lactic Dehydrogenase from rabbit muscle

Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

동의어(들):

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
9001-60-9
EC 번호:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
UNSPSC 코드:
12352204
NACRES:
NA.54

주요 문서

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유형

Type II

Quality Level

200

양식

ammonium sulfate suspension

특이 활성도

800-1,200 units/mg protein

분자량

140 kDa

외래 활성

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

배송 상태

wet ice

저장 온도

2-8°C

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일반 설명

Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

애플리케이션

L-Lactic Dehydrogenase from rabbit muscle has been used:
  • for protein-binding measurement
  • to detect the action of oxaloacetate decarboxylase
  • to determine serum L- and D-lactate

생화학적/생리학적 작용

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.

물리적 형태

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

분석 메모

Protein determined by biuret.

기타 정보

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H334

예방조치 성명서

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCF1086
SLCD5636
SLCB3953
SLBT2408
SLBP7664V

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Nutritional Biochemistry, 233-233 (1999)
Autofluorescence from NADH conformations associated with different metabolic pathways monitored using nanosecond-gated spectroscopy and spectral phasor analysis
Maltas J, et al.
Analytical Chemistry, 87(10), 5117-5124 (2015)
Anaerobic growth of Escherichia coli on D-tartrate depends on the fumarate carrier DcuB and fumarase, rather than the L-tartrate carrier TtdT and L-tartrate dehydratase
Kim OB, et al.
Archives of Microbiology, 188(6), 583-589 (2007)
José L Vasconcelos-Lima et al.
Genetics and molecular biology, 44(4), e20210127-e20210127 (2021-11-23)
Variations in dissolved oxygen levels are common in the Amazonian aquatic environments and the aquatic organisms that inhabit these environments developed a variety of adaptive responses to deal with such conditions. Some Amazonian fish species are tolerant to low oxygen
Duration of time that beef cattle are fed a high-grain diet affects the recovery from a bout of ruminal acidosis: Short-chain fatty acid and lactate absorption, saliva production, and blood metabolites
Schwaiger T, et al.
Journal of Animal Science, 91(12), 5743-5753 (2013)
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문서

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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