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Merck

L1882

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

동의어(들):

WGA, Wheat germ agglutinin

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352202
NACRES:
NA.32

주요 문서

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기술 서비스
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도움 문의

결합

agarose conjugate

Quality Level

200

양식

saline suspension

라벨링 범위

5-10 mg per mL

Matrix

cross-linked 4% beaded agarose

기질 활성

cyanogen bromide

용량

≥0.1 mg/mL binding capacity (ovomucoid)

저장 온도

2-8°C

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관련 카테고리

일반 설명

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

애플리케이션

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

생화학적/생리학적 작용

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

물리적 형태

Suspension in 1.0 M NaCl containing 15 mM sodium azide

제조 메모

Conjugates are prepared from affinity purified lectin.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

개인 보호 장비

Eyeshields, Gloves

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
109M4093V
11181032
108M4140V
118M4140V
118M4037V

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

K Paz et al.
The Journal of biological chemistry, 271(12), 6998-7003 (1996-03-22)
A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM)
Binding sugars: from natural lectins to synthetic receptors and engineered neolectins
Arnaud J, et al.
Chemical Society Reviews, 42(11), 4798-4813 (2013)

문서

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

프로토콜

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

관련 콘텐츠

Glycobiology Brochure

Glycobiology and Glycoproteomics Brochure

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.