사용
kit sufficient for 50 tests
Quality Level
포장
pkg of 1 kit
저장 조건
dry at room temperature
기술
UV/Vis spectroscopy: suitable
λmax
340 nm
응용 분야
cell analysis
detection
저장 온도
2-8°C
애플리케이션
For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
- in minimum essential medium (MEM) medium
- in the supernatant
- in plasma
생화학적/생리학적 작용
The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Tianyu Han et al.
Cell research, 28(6), 655-669 (2018-03-09)
Glutamine metabolism plays an important role in cancer development and progression. Glutaminase C (GAC), the first enzyme in glutaminolysis, has emerged as an important target for cancer therapy and many studies have focused on the mechanism of enhanced GAC expression
Yingchao Lin et al.
BMC plant biology, 17(1), 247-247 (2017-12-21)
The trehalose (Tre) pathway has strong effects on growth and development in plants through regulation of carbon metabolism. Altering either Tre or trehalose 6-phosphate (T6P) can improve growth and productivity of plants as observed under different water availability. As yet
Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism
Schop D, et al.
Journal of Tissue Engineering and Regenerative Medicine, 4(2), 131-140 (2010)
Maryvonne Ardourel et al.
Nutrients, 13(9) (2021-09-29)
To prevent ocular pathologies, new generation of dietary supplements have been commercially available. They consist of nutritional supplement mixing components known to provide antioxidative properties, such as unsaturated fatty acid, resveratrol or flavonoids. However, to date, only one preclinical study
I Made Adi Parmana et al.
Vascular health and risk management, 18, 219-231 (2022-04-13)
Myocardial injury due to on-pump coronary artery bypass grafting (CABG) in patients with low ejection fraction (EF) is associated with poor outcomes. This study determines whether intravenous glutamine could protect the myocardium during on-pump CABG in patients with low EF.
문서
Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
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