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Merck

F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, Molecular Biology, lyophilized powder

동의어(들):

Polysucrose 400, Ficoll 400

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
26873-85-8
MDL number:
MFCD00081599
UNSPSC 코드:
12352200
NACRES:
NA.52

주요 문서

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기술 서비스
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도움 문의

생물학적 소스

non-animal source

Quality Level

200

Grade

Molecular Biology

제품 라인

BioXtra

양식

lyophilized powder

외래 활성

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

저장 온도

room temp

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관련 카테고리

일반 설명

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

애플리케이션

Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).
Used for cell separation and organelle isolation.

특징 및 장점

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

기타 정보

A nonionic synthetic polymer of sucrose.

법적 정보

Ficoll is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCJ1869
SLBZ3396
SLBF5016V
091M6022V
091M6022

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리 방문

Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Weihua Fei et al.
PLoS genetics, 7(7), e1002201-e1002201 (2011-08-11)
Lipid droplets (LDs) are important cellular organelles that govern the storage and turnover of lipids. Little is known about how the size of LDs is controlled, although LDs of diverse sizes have been observed in different tissues and under different
Oindrila Bhattacharya et al.
Plant methods, 16, 131-131 (2020-09-29)
Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated
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