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Merck

F2518

Sigma-Aldrich

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

동의어(들):

Fibronectin

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

EC Number:
289-149-2
MDL number:
MFCD00131062
UNSPSC 코드:
12352202
NACRES:
NA.75

주요 문서

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제품 이름

Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture

생물학적 소스

human foreskin fibroblasts

Quality Level

200

제품 라인

BioReagent

양식

lyophilized powder

분자량

~550 kDa

포장

pkg of 0.5 mg

기술

cell culture | mammalian: suitable

표면 범위

1‑5 μg/cm2

불순물

Human Source, none detected (HCV)
 Human Source, none detected (HIV)
 Human Source, none detected (HTLV)
 Human Source, none detected (HbSAg)
 Microbial Contamination, passes test

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI 수납 번호

NM_212482.4

UniProt 수납 번호

P02751

결합 특이성

Peptide Source: Collagen

배송 상태

ambient

저장 온도

−20°C

유전자 정보

human ... FN1(2335)

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관련 카테고리

일반 설명

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

애플리케이션

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

생화학적/생리학적 작용

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

특징 및 장점

  • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
  • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
  • The starting material is tested and found negative for HIV and HBsAg.
  • The product is soluble in sterile water, yielding a clear, colorless solution.
  • It can be stored at -20°C for at least three years.

물리적 형태

Lyophilized in CAPS buffered saline

제조 메모

Store reconstituted solution in working aliquots at -20°C or lower.
dialyzed against chloroform

분석 메모

Protein homogeneity is evaluated by immunoelectrophoresis.

면책조항

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
049M4164V
11180632
087M4132V
11170515
037M4075V

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery.
Tsang T M, et al.
Infection and Immunity, 78(8), 3358-3368 (2010)
Ursula Fürnkranz et al.
Acta veterinaria Scandinavica, 55, 63-63 (2013-09-10)
The cell invasiveness of Mycoplasma gallisepticum, the causative agent of respiratory disease in chickens and infectious sinusitis in turkeys, may be a substantial factor in the well-known chronicity of these diseases and in the systemic spread of infection. To date
Regulation of matrix Accumulation (2012)
Caroline Chagnot et al.
PloS one, 8(3), e59386-e59386 (2013-03-22)
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 are responsible for repeated food-poisoning cases often caused by contaminated burgers. EHEC infection is predominantly a pediatric illness, which can lead to life-threatening diseases. Ruminants are the main natural reservoir for EHEC and food contamination
Natural variant of collagen-like protein A in serotype M3 group A Streptococcus increases adherence and decreases invasive potential.
Flores A R, et al.
Infection and Immunity (2015)
모든 관련된 서류 보기

문서

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

프로토콜

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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