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Merck

F2426

Millipore

EZview™ Red ANTI-FLAG® M2 Affinity Gel

clone M2

동의어(들):

Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
NACRES:
NA.32

주요 문서

모든 문서 보기
기술 서비스
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도움 문의
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

클론

M2, monoclonal

Quality Level

200

분석물 화학적 분류

proteins

기술

affinity chromatography: suitable
immunoprecipitation (IP): suitable

Matrix

4% agarose bead; 45-165μm bead size

동형

IgG1

용량

≥0.6 mg/mL, gel binding capacity

배송 상태

wet ice

저장 온도

−20°C

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관련 카테고리

일반 설명

EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.

애플리케이션

Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.

Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide

Learn more product details in our FLAG® application portal.

생화학적/생리학적 작용

Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.

물리적 형태

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

FLAG® Affinity Gels, FLAG® tag, 3xFLAG®tag, DYKDDDDK tag

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCL4336
SLCJ4442
SLCF7218
SLCD8965
SLCC6050

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
W Du et al.
Cell death and differentiation, 16(11), 1493-1504 (2009-07-11)
The tumor suppressor p53 induces potent anti-proliferative responses in stressed cells; in unstressed cells this ability of p53 is restrained by Hdm2. Expression of Hdm2 is also induced by p53, thereby establishing feedback inhibition. Regulation of the p53-Hdm2 interaction and
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
Matthew Frieman et al.
Journal of virology, 83(13), 6689-6705 (2009-04-17)
The outcome of a viral infection is regulated in part by the complex coordination of viral and host interactions that compete for the control and optimization of virus replication. Severe acute respiratory syndrome coronavirus (SARS-CoV) intimately engages and regulates the
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프로토콜

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

관련 콘텐츠

단백질 발현

단백질 발현 기술은 연구, 치료제, 백신 생산을 지원하는 다양한 발현 시스템에 사용됩니다.

단백질 정제

이온교환, 크기 배제 및 단백질 친화성 크로마토그래피를 포함한 방법을 사용하는 재조합형 단백질 정제를 위한 단백질 정제 기법, 시약 및 프로토콜.

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

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