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Merck

E5036

Sigma-Aldrich

Epidermal Growth Factor Protein, human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

동의어(들):

Epidermal Growth Factor human, EGF

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
62253-63-8
MDL number:
MFCD00132565
UNSPSC 코드:
12352202
NACRES:
NA.77

주요 문서

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제품 이름

Epidermal Growth Factor, human, animal component free, EGF, recombinant, expressed in Escherichia coli, >97% (SDS-PAGE)

생물학적 소스

human

Quality Level

200

재조합

expressed in E. coli

분석

>97% (SDS-PAGE)

양식

lyophilized powder

효능

0.08-0.8 ng/mL EC50

분자량

~6 kDa

포장

pkg of 200 and 500 μg

저장 조건

avoid repeated freeze/thaw cycles

불순물

≤1 EU/μg Endotoxin

색상

white

solubility

water: soluble 0.190-0.210, clear, colorless

UniProt 수납 번호

P01133

저장 온도

−20°C

SMILES string

S(CC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]%12N(CCC%12)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](N

InChI

1S/C270H401N73O83S7/c1-24-134(19)217(262(420)293-112-201(355)298-161(69-74-205(359)360)229(387)301-160(45-35-82-286-269(279)280)228(386)333-192(119-428)255(413)307-162(68-73-198(274)352)230(388)316-176(94-141-54-64-150(350)65-55-141)241(399)304-159(44-34-

InChI key

GVUGOAYIVIDWIO-UFWWTJHBSA-N

유전자 정보

human ... EGF(1950)

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관련 카테고리

일반 설명

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.† Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.† EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.† EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,† which appears to utilize the EGF receptor to gain entry into cell† EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.†
Epidermal growth factor (EGF) is synthesized in Henle′s loop and the distal convoluted tubule in the kidney. It is also produced in salivary glands and duodenum.

애플리케이션

Epidermal Growth Factor, human, animal component free has been used:
  • as a supplement in in LHC-8 medium to culture liver cell lines
  • in the fetal bovine serum (FBS)-Dulbecco′s modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells
  • as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cells
  • as a component in tumorsphere medium

생화학적/생리학적 작용

Epidermal growth factor (EGF) helps to induce cell growth, proliferation and differentiation. It participates in the repairing of renal tissues in the kidney. It promotes the reabsorption of magnesium with the help of the transient receptor potential cation channel 6 (TRMP6). EGF is known to participate in the pathophysiology of drug-induced renal magnesium loss.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
059M4058V
059M4057V
118M4121V
117M4079V
117M4078V

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Epidermal growth factor and its influencing variables in healthy children and adults
Meybosch S, et al.
Testing, 14(1), e0211212-e0211212 (2019)
Cancer-associated fibroblasts induce epithelial-mesenchymal transition through secreted cytokines in endometrial cancer cells
Wang X, et al.
Oncology Letters, 15(4), 5694-5702 (2018)
In vitro tumorsphere formation assays
Johnson S, et al.
Bio-protocol, 3(3), e325-e325 (2013)
Gab3 overexpression in human glioma mediates Akt activation and tumor cell proliferation
Jia P, et al.
Testing, 12(3), e0173473-e0173473 (2017)
Isako Saga et al.
Neuro-oncology, 16(8), 1048-1056 (2014-05-27)
The metabolic preference of malignant glioma for glycolysis as an energy source is a potential therapeutic target. As a result of the cellular heterogeneity of these tumors, however, the relation between glycolytic preference, tumor formation, and tumor cell clonogenicity has
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