B9673
1-Bromo-3-chloropropane
for isolation of RNA
동의어(들):
1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
로그인조직 및 계약 가격 보기
크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
Cl(CH2)3Br
CAS 번호:
Molecular Weight:
157.44
Beilstein:
605278
EC Number:
MDL number:
UNSPSC 코드:
41105515
PubChem Substance ID:
NACRES:
NA.52
Grade:
Molecular Biology
Bp:
144-145 °C (lit.)
일반 설명
BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.
애플리케이션
1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
- feline teeth
- mice brain and liver tissues
- human ectocervical epithelial cells
- placental samples
- human mesenchymal stromal cells (hMSCs)
특징 및 장점
- less toxic alternative to chloroform
- compatible with TRI reagent for isolation of RNA with decreased DNA contamination
기타 정보
BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
개인 보호 장비
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
Lashkari BS and Anumba DOC
PLoS ONE, 12(3), e0173646-e0173646 (2017)
Ghalia Boubaker et al.
PLoS neglected tropical diseases, 14(5), e0007640-e0007640 (2020-05-23)
We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection of experimental primary alveolar echinococcosis (AE), a parasitic infection caused upon ingestion of E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice
Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation.
P Chomczynski et al.
Analytical biochemistry, 225(1), 163-164 (1995-02-10)
Isolation of total RNA from transgenic mouse melanoma subsets using fluorescence-activated cell sorting
Microarray methods for drug discovery, 27-44 (2010)
Neuroprotection Induced by Energy and Protein-Energy Undernutrition Is Phase-Dependent After Focal Cerebral Ischemia in Mice
de Carvalho TS, et al.
Translational Stroke Research, 1-12 (2019)
프로토콜
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
TRI 시약은 DNA, RNA 및 단백질을 동시에 분리할 수 있게 하는 단일 과정의 RNA 분리 시약입니다. 이 프로토콜은 시료 제조 및 다양한 시료 추출, 문제 해결의 개요를 제공합니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.