일반 설명
Protein determination is one of the most common operations performed in biochemical research. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. Both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. The BCA assay is more sensitive and applicable than either biuret or Lowry procedures.
애플리케이션
Bicinchoninic Acid solution (Bicinchoninic acid protein assay kit) is used :
- For the determination of total protein content in the process of quantifying bromelain, extracted from the pineapple stem residues using aqueous micellar two-phase systems composed of ionic liquids as co-surfactants.
- For the determination of bovine serum albumin (BSA) in biomimetic calcium phosphate coating co-precipitated on titanium alloy plates.
This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.
특징 및 장점
- It is easy to use.
- The color complex is stable.
- There is less susceptibility to detergents.
- It is applicable over a broad range of protein concentrations.
포장
Also available as part of the Bicinchoninic Acid Kit for Protein Determination (BCA-1).
신호어
Warning
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Met. Corr. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Diego García-Ayuso et al.
Experimental eye research, 161, 10-16 (2017-05-30)
In this work we study the effects of an acute light-induced retinal degeneration on the population of melanopsin positive retinal ganglion cells (m
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente AF, et al.
Process Biochemistry (Oxford, United Kingdom), 51(4), 528-534 (2016)
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프로토콜
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
관련 콘텐츠
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
다양한 샘플 내의 단백질 농도를 정확히 측정하기 위한 단백질 정량법, 시약 및 면역분석 기술.
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.