A2765
Azocasein
protease substrate, chromogenic, powder
동의어(들):
Sulfanilamide-azocasein
제품 이름
Azocasein, protease substrate
생물학적 소스
bovine
Quality Level
양식
powder
기술
ligand binding assay: suitable
solubility
water: 5 mg/mL, clear, orange to very deep orange
ε (흡광계수)
≥25 at 440 nm in 0.1 M NaOH at 1%
저장 온도
2-8°C
일반 설명
Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.
애플리케이션
Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
기타 정보
View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
Recombinant proteins from plants: production and isolation of clinically useful compounds, 3 (1998)
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International, 2016 (2016)
Prevalence and characterization of Bacillus cereus group from various marketed dairy products in India
Kumari S and Sarkar PK
Dairy science & technology, 94(5), 483-497 (2014)
Hongfei Liu et al.
Protein and peptide letters, 27(11), 1102-1113 (2020-03-21)
Protein drugs have disadvantages, such as short half-lives, unstable biological activities, and low utilization efficiency. In this paper, a porous ion-responsive targeted drug delivery system was designed, combining biodegradable carriers with ion exchange technology to overcome problems for protein drug
Azocasein assay for alkaline protease in complex fermentation broth
Iversen SL and Jorgensen MH
Biotechnol. Tech., 9(8), 573-576 (1995)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.