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WGA4

GenomePlex® Single Cell Whole Genome Amplification Kit

Amplify genome of a single cell

동의어(들):

Single cell whole genome amplification, Whole genome amplification

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352200
EC Number:
254-457-8
NACRES:
NA.55
기술 서비스
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도움 문의

Quality Level

200

technique(s)

whole genome amplification: suitable

dilution

(WGA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

General description

GenomePlex® Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. This kit is designed to amplify the genome of a single cell. This rapid and straightforward method provides millionfold amplification yielding microgram quantities of genomic DNA from a single cell. Traditional single-cell whole genome amplification methods yield insufficient quantities with significantly biased representation. The kit includes all the reagents necessary for cell lysis and successive whole genome amplification. Single cells can be isolated by fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), dilution, or any other applicable method.

Application

GenomePlex® Single Cell Whole Genome Amplification Kit has been used:

  • to amplify the isolated DNA
  • in whole genome amplification (WGA)
  • to amplify the microdissected DNAs
  • for the amplification of circulating tumor cell genomic DNA from metastatic castration-resistant prostate cancer cells
  • for the detection of copy number variations (CNV) by single-cell low-coverage whole-genome sequencing (SLWGS) method
  • Gel electrophoresis
  • qPCR
  • comparative genomic hybridization (CGH) microarray
  • Short tandem repeats (STR) analysis
  • single nucleotide polymorphism (SNP) analysis

Features and Benefits

  • Highly yield and accuracy of DNA amplification within four hours
  • Amplification of DNA from any source such as cancer cells, epithelial cells, lymphocytes, fibroblast amniotic cells, polycarbonate fixed cells, and plant cells
  • A complete representation of the entire genome with minimal allele bias
  • WGA DNA polymerase is suitable for use with downstream applications including gel electrophoresis, qPCR, comparative genomic hybridization (CGH) microarray, short term repeat (STR) analysis, and single nucleotide polymorphism (SNP) analysis, TaqMan® assays, and microsatellite analysis

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS & 가격
  • L1043
    10x Single Cell Lysis & Fragmentation Buffer
    SDS
  • P4850
    Proteinase K from Tritirachium album, buffered aqueous glycerol solution, Molecular Biology, ≥800 units/mL
    SDS
  • W4502
    Water, Nuclease-Free Water, for Molecular Biology
    SDS

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

저장 등급

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
097K1681
097K1682
037K0447
037K0448
115K0427

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COA 검색

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GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol

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문서

Whole Genome Amplification for Single Cell Biology

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

관련 콘텐츠

Whole Genome Amplification Product Listing

Our commitment to the Life Sciences continues through significant advances in PCR technology. Improvements to the key parameters of yield, length, specificity, and fidelity, as well as new applications for nucleic acid amplification form the basis of our research and development focus. We are dedicated to providing you with the newest technology as the field of Nucleic Acid Amplification evolves, making Sigma your preferred supplier for PCR products.

Single Cell Aneuploidy Analysis by High Resolution Comparative Genomic Hybridization

Single Cell Aneuploidy Analysis by High Resolution Comparative Genomic Hybridization (CGH) after Whole Genome Amplification reveals Maternal Cell Contamination in the Original Products of Conception Cultures.

Biodiscover Whole Genome Amplification Advisor

Whole Genome Amplification Advisor

관련 콘텐츠 모두 보기
Nathan R Treff et al.
Molecular human reproduction, 16(8), 583-589 (2010-05-21)
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods of aneuploidy screening of blastomeres within the same embryo has not been conducted and may indicate whether mosaicism has been overestimated
Nathan R Treff et al.
Fertility and sterility, 94(6), 2017-2021 (2010-03-02)
To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. Prospective, randomized, and blinded study. Academic reproductive medicine center. Multiple euploid and aneuploid cell lines were obtained
Resolving tumor heterogeneity: genes involved in chordoma cell development identified by low-template analysis of morphologically distinct cells
El-Heliebi A, et al.
PLoS ONE, 9(2), e87663-e87663 (2014)
모든 관련된 서류 보기

국제 무역 품목 번호

SKUGTIN
WGA4-10RXN04061835378777
WGA4-500RXN04061835378784
WGA4-50RXN04061835378791