Quality Level
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
General description
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
프로토콜
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
문서
Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.
관련 콘텐츠
This protocol describes the use of MISSION TRC shRNA Lentiviral Particles and provides a system for long-term silencing and phenotypic observation.
Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (-)-Gossypol (AT-101).
Mani J
BMC Cancer, 15, 224-224 (2015)
Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor
Nature, 099655-099655 (2017)
Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.
Miura N
Oncotarget (2016)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| SHC001V | 04061837018213 |
| SHC001VN | 04065269394297 |