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S1797

SOC Medium

For use in transformation

동의어(들):

Super Optimal broth

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.85
UNSPSC Code:
41106200
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도움 문의

grade

Molecular Biology

Quality Level

500

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number
088K8415
047K8423
085K8410
104K8410
109H8413

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문서 라이브러리 방문

프로토콜

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

문서

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

관련 콘텐츠

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mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

Directional Cloning System
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Meg L Flanagan et al.
Methods in molecular biology (Clifton, N.J.), 378, 33-52 (2008-07-09)
As a source of recombinant antigen, soluble constant fragment (Fc) fusion proteins have become valuable reagents for immunotherapy and laboratory investigations. Additional applications for these reagents include flow cytometry, immunohistochemistry, and in vitro activity assays. To aid investigators in the
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국제 무역 품목 번호

SKUGTIN
S1797-10X5ML04061838262424
S1797-100ML04061838262417
S1797-10ML04061838080356
S1797-5ML04061838108456