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QR0200

Quantitative RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

동의어(들):

1-step RT-qPCR mix, Quantitative real-time PCR master mix

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.55
UNSPSC Code:
41106300
기술 서비스
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도움 문의

Quality Level

300

usage

sufficient for 250 reactions

feature

dNTPs included, hotstart

technique(s)

RT-qPCR: suitable, qPCR: suitable

color

colorless

input

purified RNA

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

General description

Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Features and Benefits

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Legal Information

This product is for research use only.
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

제품 번호
설명
  • Probe Based qRT-PCR ReadyMix 2 X
  • Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS & 가격
  • P2192
    10X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vial
    SDS
  • M8787
    Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mM
    SDS
  • P2192
    10X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 X
    SDS

pictograms

Exclamation markEnvironment
GHS07,GHS09

저장 등급

12 - Non Combustible Liquids

wgk

WGK 3

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
026K6837
115K6027
065K6104
054K6007
084K6109

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문서 라이브러리 방문

프로토콜

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

문서

mRNA Synthesis for the Development of Vaccines and Therapeutics

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GC5, GC10, and Thunderbolt Competent Cells
관련 콘텐츠 모두 보기
Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., 22-32 (2002)
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can

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SKUGTIN
QR0200-1KT04061835516742