Protein G−Peroxidase from Streptococcus sp.

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H₂O₂ homeostasis in cells. It aids the regulation of ABC Mn²⁺-permease complex (psaBCA) genes.

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

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