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Merck

L2542

LB Broth (Miller)

Liquid microbial growth medium

동의어(들):

Luria Broth Miller, Luria-Bertani broth, Miller

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.85
UNSPSC Code:
41106200
기술 서비스
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도움 문의


grade

Molecular Biology

Quality Level

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Other Notes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract


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저장 등급

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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문서 라이브러리 방문


프로토콜

General protocols for growth of competent cells in microbial medium.

문서

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

유전 공학 및 복제의 발전은 연구 목적을 위한 이형 Protein Expression Systems 및 분리에 수많은 가능성을 열어주었습니다. 상당한 수준의 기술 발전으로 재조합형 단백질의 대규모 발현과 분리가 가능하게 되었습니다.


D O Chaffin et al.
Journal of bacteriology, 187(13), 4615-4626 (2005-06-22)
Several bacterial pathogens have evolved the means to escape immune detection by mimicking host cell surface carbohydrates that are crucial for self/non-self recognition. Sialic acid, a terminal residue on these carbohydrates, inhibits activation of the alternate pathway of complement by
Thomas P Burg et al.
Nature, 446(7139), 1066-1069 (2007-04-27)
Nanomechanical resonators enable the measurement of mass with extraordinary sensitivity. Previously, samples as light as 7 zeptograms (1 zg = 10(-21) g) have been weighed in vacuum, and proton-level resolution seems to be within reach. Resolving small mass changes requires
Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)



국제 무역 품목 번호

SKUGTIN
L2542-500ML04061833950180