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Merck

GE17-0851-01

Disposable PD 10 Desalting Columns

Cytiva 17-0851-01, pack of 30 pieces

동의어(들):

Disposable desalting columns, PD-10 columns

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.56
UNSPSC Code:
23151816
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form

prepacked column

crosslinking

cross-linked

feature

wetted part: no

packaging

pack of 30 pieces

manufacturer/tradename

Cytiva 17-0851-01

storage condition

(0.15% kathon)

parameter

2.5 mL sample volume

technique(s)

liquid chromatography (LC): suitable

bed size

14.5 mm × 50 mm

bed volume

8.3 mL

column I.D.

15 mm

matrix

cross-linked dextran

matrix active group

dextran

particle size

50-150 μm

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

General description

Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.

The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.

Features and Benefits

  • Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex® G-25 Medium.
  • Flexible protocols allow runs by gravity flow or centrifugation.
  • Easy to use and equilibrate; apply sample; elute.
  • Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
  • Desalt multiple samples in parallel with a desalting capacity >90%.
  • Recovery in the range 70 to >95%.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Legal Information

Sephadex is a registered trademark of Cytiva


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Warning

hcodes

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문


프로토콜

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

모든 프로토콜 보기

문서

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

모든 기사 보기

Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell



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SKUGTIN
GE17-0851-0104061835573127