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E1014

Benzonase^® Nuclease

Name: Benzonase<SUP>®</SUP> Nuclease
Brand: SIGMA

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

동의어(들):

Endonuclease from Serratia marcescens

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9025-65-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC 번호:

3.1.30.2 (BRENDA, IUBMB)

MDL number:

MFCD00131010

Assay:

≥90% (SDS-PAGE)

Biological source:

Serratia marcescens

Recombinant:

expressed in E. coli

Concentration:

≥250 units/μL

기술 서비스

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도움 문의

속성

biological source

Serratia marcescens

Quality Level

200

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

30 kDa

concentration

≥250 units/μL

application(s)

research use

foreign activity

protease, essentially free

shipped in

wet ice

storage temp.

−20°C

설명

General description

Benzonase^® nuclease is a highly efficient and genetically engineered endonuclease that originates from Serratia marcescens. This dimeric protein with two essential disulfide bonds is capable of attacking and degrading all forms of DNA and RNA (single-stranded, double-stranded, linear, and circular) under a wide range of operating conditions. Benzonase^® nuclease is capable of removing nucleic acids and enhancing the purity and quality of protein samples.

Application

Benzonase^® Nuclease has been used: as a component in ice-cold lysis buffer C to digest DNA and  RNA to facilitate the complete release of all nuclear proteins in the immunoprecipitation step to release protein complexes from the nucleoplasm and chromatinas a supplement in RIPA to fractionate SHSY5Y cells for immunoprecipitation to remove residual nucleic acids from the aortic roots in decellularization method

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Benzonase^® Nuclease can completely digest nucleic acids into 5′-monophosphate terminated oligonucleotides of 3 to 5 bases in length, making it the ideal tool for removing nucleic acids from recombinant proteins and for applications that require complete digestion of nucleic acids. In addition to reducing viscosity in protein extracts and preventing cell clumping, pretreatment of protein samples with Benzonase® nuclease can significantly improve their resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. This versatile enzyme can digest both native or heat-denatured DNA and RNA, with its optimum pH for enzyme activity found to be 8.0-9.2. Benzonase^® nuclease is effective at removing host DNA from microbiome samples. In many cases, microbiome samples (such as saliva or skin) will have a high percentage of host DNA that interferes with downstream results. Our experts show that the reduction of host DNA lowers the cost of sequencing while increasing and improving the data. Experimental data is shown in the technical article - Benzonase^® Nuclease for Microbiome Workflows

Digests native or heat-denatured DNA and RNA.

Features and Benefits

Host DNA depletion in microbiome samples.

Effective nucleic acid digestion in a variety of workflows.

Viscosity reduction during protein extraction.

Physical form

Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl₂, and 20 mM NaCl.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).

Legal Information

Benzonase^® Nuclease is supplied by Merck KGaA, Darmstadt, Germany and/or its affiliates.

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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문서

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

Benzonase^® Nuclease for Microbiome Workflows

Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.

모든 기사 보기

국제 무역 품목 번호

SKU	GTIN
E1014-25KU	04061835572915
E1014-5KU	04061835572922