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Merck

C7902

Calcium chloride dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
CaCl2 · 2H2O
CAS 번호:
Molecular Weight:
147.01
NACRES:
NA.71
PubChem Substance ID:
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
Assay:
≥99.0%
Form:
powder
Solubility:
H2O: 294 mg/mL, clear, colorless
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product line

BioReagent

Quality Level

assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

General description

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Application

Calcium chloride dihydrate has been used:
  • in the formulation of media
  • in sperm collection and preparation
  • in cell culture and protoplast preparation



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Hazard Classifications

Eye Irrit. 2

저장 등급

13 - Non Combustible Solids

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WGK 1

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Not applicable

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문서 라이브러리 방문


문서

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.


The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes
PANCARCI S M, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16(5) (2010)
Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
Marco Wallenborn et al.
PloS one, 15(7), e0234986-e0234986 (2020-07-08)
Glioblastoma is a common, malignant brain tumor whose disease incidence increases with age. Glioblastoma stem-like cells (GSCs) are thought to contribute to cancer therapy resistance and to be responsible for tumor initiation, maintenance, and recurrence. This study utilizes both SNP



국제 무역 품목 번호

SKUGTIN
C7902-500G04061835543540
C7902-1KG04061835543533