제품 이름
SIGMAFAST™ BCIP®/NBT, tablet
form
tablet
Quality Level
solubility
water: 10 mL, clear, yellow
storage temp.
−20°C
Application
SIGMAFAST BCIP®/NBT has been used as a substrate
- for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
- for alkaline phosphatase in the histochemical staining of primary osteoblasts
- for alkaline phosphatase staining in bone marrow stromal cells
Preparation Note
Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
저장 등급
11 - Combustible Solids
프로토콜
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Andriy Didovyk et al.
ACS synthetic biology, 6(12), 2198-2208 (2017-08-11)
Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| B5655-5TAB | 04061835553952 |
| B5655-25TAB | 04061835553945 |