Quality Level
form
emulsion (aqueous)
technique(s)
cell culture | hybridoma: suitable, microbiological culture: suitable
density
0.97 g/mL at 25 °C
application(s)
microbiology
storage temp.
room temp
suitability
(Mammalian (suspension)), bacteria (fermentation)
General description
Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.
Application
Antifoam A concentrate has been used:
- in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.
- to prevent foam formation during the aerobic treatment of sludge.
- to study the impact of surface tension on bioaerosol generation during bubbling.
Features and Benefits
- Molecular biology grade and tested for use in bacterial fermentation.
- Extremely effective foam suppressor for aqueous and non-aqueous systems.
- Made of 100% active silicone polymer, free of emulsifiers.
- Typically, effective at 1-100 ppm.
- Product will be stable in the pH range of 5 to 9.
- Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.
Preparation Note
Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.
Other Notes
Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
>213.8 °F - closed cup
flash_point_c
> 101 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - PBT - vPvB
M Schlömann et al.
Journal of bacteriology, 172(9), 5119-5129 (1990-09-01)
Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase from Alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-4-fluoro-but-2-en-4-olide]. This compound was shown to
F P Ernani et al.
Infection and immunity, 61(6), 2596-2601 (1993-06-01)
Substantial evidence indicates that molecules released by infectious organisms affect virulence and influence immunity to infection. The characterization of extracellular molecules and their mechanism of release is therefore critical to understanding host-parasite relationships. The cestode parasite Mesocestoides corti is known
Experience with a perfo-suction system for the recovery of schistosomes from laboratory rodents
T. F. H. G. Jackson, C. D. Dettman, et al.
Laboratory Animal Science, 16, 65-67 (1982)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| A6582-100G | 04061833382516 |