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Merck

79760

Sigma-Aldrich

Phthaldialdehyde

suitable for fluorescence, ≥99.0% (HPLC)

동의어(들):

Benzene-1,2-dicarboxaldehyde, OPA, o-Phthalaldehyde, o-Phthalic dicarboxaldehyde, o-Phthalaldehyde, o-Phthalic dicarboxaldehyde

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C8H6O2
CAS 번호:
643-79-8
Molecular Weight:
134.13
Beilstein:
878317
EC Number:
211-402-2
MDL number:
MFCD00003335
UNSPSC 코드:
12352201
PubChem Substance ID:
57652816
NACRES:
NA.32

주요 문서

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Quality Level

100

분석

≥99.0% (HPLC)

양식

solid

분자량

134.13 g/mol

농도

≤100% (o-phthaldialdehyde)

불순물

≤0.5% phthalic acid

색상

white to light yellow

bp

83 °C/1.067 hPa

mp

54-56 °C

solubility

ethanol: 0.5 g/10 mL, clear, colorless to greenish-yellow
water: 37.7 g/L at 20 °C (OECD Test Guideline 105)

density

1.13 g/cm3 at 20 °C

형광

λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)

적합성

suitable for fluorescence

저장 온도

2-8°C

SMILES string

O=Cc1ccccc1C=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

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일반 설명

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.

애플리케이션

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC. Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.

생화학적/생리학적 작용

Phthaldialdehyde can be used for the pre-column derivatization of amino acids for high-performance liquid chromatography (HPLC) separation. It may also be used for flow cytometric measurements of protein thiol groups.

픽토그램

Skull and crossbonesCorrosionEnvironment
GHS06,GHS05,GHS09

신호어

Danger

유해 및 위험 성명서

H301,H314,H317,H335,H410

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

269.6 °F - closed cup

Flash Point (°C)

132 °C - closed cup

개인 보호 장비

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
BCCF5794
BCCC3481
BCCB5919
BCCB2021
BCBW3354

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문서 라이브러리 방문

José María Landete et al.
International journal of food microbiology, 115(3), 364-368 (2007-02-20)
The ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. An easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific PCR assay that detects the presence
Hanne Vissenaekens et al.
Food research international (Ottawa, Ont.), 145, 110430-110430 (2021-06-12)
Flavonoid bioavailability and bioactivity is associated with interindividual variability, which is partially due to differences in health status. Previously, it was demonstrated that cellular stress, especially mitochondrial stress, increases intracellular quercetin uptake and this is associated with beneficial health effects.
Yun-Bin Han et al.
Applied microbiology and biotechnology, 99(16), 6715-6726 (2015-02-17)
Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
Niklas Krause et al.
Scandinavian journal of work, environment & health, 41(2), 124-139 (2015-01-20)
This study aimed to assess the effects of physically demanding work - measured as energy expenditure (EE) during occupational physical activities (OPA) - on risk of acute myocardial infarction (AMI) among men with and without preexisting ischemic heart disease (IHD).
M Nurul Islam et al.
The New phytologist, 193(1), 51-57 (2011-11-11)
• Sphingolipids are emerging as important mediators of cellular and developmental processes in plants, and advances in lipidomics have yielded a wealth of information on the composition of plant sphingolipidomes. Studies using Arabidopsis thaliana showed that the dihydroxy long-chain base
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