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N6639

Nitrotetrazolium Blue chloride

Name: Nitrotetrazolium Blue chloride
Brand: SIAL

powder, electrophoresis grade

동의어(들):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

C₄₀H₃₀N₁₀O₆ · 2Cl

CAS 번호:

298-83-9

Molecular Weight:

817.64

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

24897786

EC Number:

206-067-4

Beilstein/REAXYS Number:

4115923

MDL number:

MFCD00012159

기술 서비스

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도움 문의

속성

grade

electrophoresis grade

Quality Level

100

assay

>90%

form

powder

technique(s)

electrophoresis: suitable

solubility

H₂O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.), 2-methoxyethanol: 20 mg/mL, ethanol: 5 mg/mL

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

설명

General description

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Application

Nitrotetrazolium Blue chloride has been used:

to determine the superoxide anion in skeletal muscle cells
as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections
as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O₂- ) anion in Brassica juncea seedlings
as a component in Triton X-100 solution to perform staining of coronal brain sections

Biochem/physiol Actions

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O₂.

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pictograms

GHS07

signalword

Warning

hcodes

H302,H315,H319,H335

pcodes

P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Histochemical detection of superoxide and H2O2 accumulation in Brassica juncea seedlings

Kumar D, et al.

Bio-protocol, 4(8), e1108-e1108 (2014)

The systemDrive: a Multisite, Multiregion Microdrive with Independent Drive Axis Angling for Chronic Multimodal Systems Neuroscience Recordings in Freely Behaving Animals

Billard MW, et al.

eNeuro, 5(6) (2018)

Ontogenesis of NADPH-diaphorase positive neurons in guinea pig neocortex

Liu C, et al.

Frontiers in Neuroanatomy, 9 (2015)

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국제 무역 품목 번호

SKU	GTIN
N6639-1G	04061835574445
N6639-250MG	04061835574452
N6639-50MG	04061835574469