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E4884

Ethylenediaminetetraacetic acid disodium salt dihydrate

Name: Ethylenediaminetetraacetic acid disodium salt dihydrate
Brand: SIAL

ACS reagent, 99.0-101.0%

동의어(들):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₁₀H₁₄N₂Na₂O₈ · 2H₂O

CAS 번호:

6381-92-6

Molecular Weight:

372.24

NACRES:

NA.77

PubChem Substance ID:

24894586

UNSPSC Code:

12352107

EC Number:

205-358-3

Beilstein/REAXYS Number:

3900609

기술 서비스

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도움 문의

속성

Quality Level

200

grade

ACS reagent

agency

suitable for SM 4500 - NH3

assay

99.0-101.0%

form

solid

reaction suitability

reagent type: chelator

impurities

≤0.005% Insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

pH

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

solubility

H₂O: 100 g/L at 20 °C

cation traces

Fe: ≤0.01%, heavy metals: ≤0.005% (by ICP-OES)

application(s)

microbiology

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

설명

General description

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Features and Benefits

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Other Notes

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pictograms

GHS08,GHS07

signalword

Warning

hcodes

H332,H373

pcodes

P260 - P271 - P304 + P340 + P312 - P314 - P501

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

저장 등급

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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국제 무역 품목 번호

SKU	GTIN
E4884-100G	04061835544486
E4884-500G	04061835544493