biological source
algae (marine)
Quality Level
form
suspension
technique(s)
affinity chromatography: suitable
bead diameter
45-165 μm
pore size
30,000-50,000 Da fractionation range (Dextrans), 60,000-20,000,000 Da fractionation range (Globular proteins)
storage temp.
2-8°C
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
General description
Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.
Application
Sepharose™ 4B has been used:
- to conjugate scorpion venom to be used in affinity chromatography
- for clearing of protein extracts prior to immunoprecipitation
- for gel purification and desalting of phospholipid vesicles
- for adsorption of immunoglobulin G (IgG)
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
저장 등급
3 - Flammable liquids
wgk
WGK 1
flash_point_f
100.4 - 109.4 °F - closed cup
flash_point_c
38 - 43 °C - closed cup
Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations
Adamala K, et al.
Nature Protocols, 10(6), 927-927 (2015)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
Hosseini Z, et al.
Iranian Journal of Toxicology, 11(2), 15-19 (2017)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 4B200-100ML | 04061832422220 |
| 4B200-1L | 04061832422237 |
| 4B200-500ML | 04061832422244 |