Chloroform

USP/NF, reag. ISO, reag. Ph. Eur.

4.1 (vs air)

160 mmHg ( 20 °C)

puriss. p.a.

99.0-99.4% (GC)

liquid

~1% ethanol as stabilizer

RNA extraction: suitable

≤0.00001% free chlorine (Cl), ≤0.00005% free acid (as HCl), ≤0.0005% non-volatile matter, ≤0.005% aldehydes and ketones (as CH₃COCH₃), ≤0.005% carbonyl compounds (as CO), ≤0.01% tetrachloroethene (GC), ≤0.01% tetrachloromethane (GC), ≤0.01% trichloroethene (GC), ≤0.01% water (Karl Fischer), ≤0.03% dichloromethane (GC), 0.6-1.0% ethanol (GC)

n20/D 1.445 (lit.)

60.5-61.5 °C (lit.)

−63 °C (lit.)

1.476-1.483 g/mL at 20 °C, 1.492 g/mL at 25 °C (lit.)

chloride (Cl^-): ≤0.0001%

ClC(Cl)Cl

Al: ≤0.50 ppm, B: ≤0.02 ppm, Ba: ≤0.10 ppm, Ca: ≤0.50 ppm, Cd: ≤0.05 ppm, Co: ≤0.02 ppm, Cr: ≤0.02 ppm, Cu: ≤0.02 ppm, Fe: ≤0.10 ppm, Mg: ≤0.10 ppm, Mn: ≤0.02 ppm, Ni: ≤0.02 ppm, Pb: ≤0.05 ppm, Sn: ≤0.10 ppm, Zn: ≤0.10 ppm

complies for appearance, complies for reaction against H₂SO₄, complies for suitability of determ. w. dithizone

1S/CHCl3/c2-1(3)4/h1H

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