Digoxigenin-11-dUTP, alkali-labile

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP), alkali-labile is provided as in 1 mM tetralithium salt, solution. In which DIG is bound to dUTP via an alkali-sensitive ester linkage.

Digoxigenin-11-dUTP, alkali-labile can be used for non-radioactive DNA labeling e.g. random primed or nick translation. It can also be used for labeling probes which are preferred in hybridization experiments where stripping and reprobing of the membrane is intended.

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphophate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

For life science research only. Not for use in diagnostic procedures.
When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will be removed. It is therefore ideal for use in DIG-labeled probes that can be removed by alkali denaturation (e.g ., in stripping and reprobing of blots). However, if you intend to make a DIG-labeled DNA that will survive alkaline treatment, do not use this alkali-labile preparation. Instead, use the alkali-stable form of DIG-11-dUTP.