생물학적 소스
fish (sperm)
Quality Level
Grade
Molecular Biology
양식
solution (ready-to-use)
분자량
40-1,000 kDa
포장
pkg of 500 mg
제조업체/상표
Roche
농도
100 μg/mL
색상
light yellowopalescent
solubility
water: miscible
저장 온도
−20°C
일반 설명
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
애플리케이션
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
물리적 형태
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
제조 메모
Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
기타 정보
Chain Length 120 to 3,000 nucleotides
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
Yu Sasano et al.
Bio-protocol, 7(10), e2306-e2306 (2017-05-20)
Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the splitting efficiency of
Dilip Kumar et al.
Journal of immunology (Baltimore, Md. : 1950), 182(2), 1011-1020 (2009-01-07)
The MAPKs ERK, JNK, and p38 control diverse aspects of the immune response, including regulation of cytotoxin biology in NK cells and CTL. The chemokine CCL5 is coreleased with the cytotoxins, perforin, the granzymes, and granulysin, during the lethal hit
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