생물학적 소스
human plasma
Quality Level
무균
non-sterile; 0.2 μm filtered
분석
95% (SDS-PAGE)
양식
lyophilized (clear, colorless solution after reconstitution)
분자량
440 kDa
포장
pkg of 5 mg
제조업체/상표
Roche
저장 조건
avoid repeated freeze/thaw cycles
기술
cell culture | mammalian: suitable
UniProt 수납 번호
저장 온도
2-8°C
유전자 정보
human ... FN1(2335)
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일반 설명
Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
애플리케이션
Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.
Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)
생화학적/생리학적 작용
Active on most mammalian cells
물리적 형태
Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.
제조 메모
Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.
기타 정보
For life science research only. Not for use in diagnostic procedures.
면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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문서
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
프로토콜
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
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