10775835001
Roche
Bovine Serum Albumin
98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin
동의어(들):
BSA, Bovine Serum Albumin Fraction V, fatty acid free, Bovine albumin, bovine serum
생물학적 소스
bovine
Quality Level
분석
98.5% (electrophoresis)
양식
lyophilized
분자량
Mr 68 kDa
포장
pkg of 50 g
제조업체/상표
Roche
기원
USA origin
기술
immunofluorescence: suitable
ligand binding assay: suitable
불순물
Immunoglobulins, none detected
Triglycerides, none detected
≤0.2 mg/g fatty acids
UniProt 수납 번호
배송 상태
wet ice
저장 온도
2-8°C
유전자 정보
bovine ... ALB(280717)
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일반 설명
Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.
애플리케이션
Bovine Serum Albumin, Fraction V, fatty acid free is used:
- in lipid research to study lipid metabolism in biological systems
- to identify transport mechanisms in living organisms
- to study ligand binding
- to investigate ionic binding
- in the preparation of solutions during isolation of cardiomyocytes
- as a blocking solution to incubate embryos for immunofluorescence
- in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers
생화학적/생리학적 작용
The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
특징 및 장점
- Free from fatty acids according to current quality control procedures (suitable for lipid research)
- High quality
- Low content of heavy metal ions
- Long-term stability
분석 메모
Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Putnam F
The Plasma Proteins: Structure, Function, and Genetic Control, 5 (2012)
Examination of the Interaction between a Membrane Active Peptide
and Artificial Bilayers by Dual Polarisation Interferometry
and Artificial Bilayers by Dual Polarisation Interferometry
Payne JAE, et al.
Bio-protocol, 7(1), 1-12 (2017)
Naomi Dicks et al.
Biology of reproduction, 105(1), 76-86 (2021-04-24)
Conditions of impaired energy and nutrient homeostasis, such as diabetes and obesity, are associated with infertility. Hyperglycemia increases endoplasmic reticulum stress as well as oxidative stress and reduces embryo development and quality. Oxidative stress also causes deoxyribonucleic acid damage, which
Relief of endoplasmic reticulum stress enhances DNA damage repair and improves development of pre-implantation embryos
Dicks N, et al.
PLoS ONE, 12(11), e0187717-e0187717 (2017)
Jelena Branovets et al.
American journal of physiology. Heart and circulatory physiology, 305(4), H506-H520 (2013-06-25)
Disruption of the creatine kinase (CK) system in hearts of CK-deficient mice leads to changes in the ultrastructure and regulation of mitochondrial respiration. We expected to see similar changes in creatine-deficient mice, which lack the enzyme guanidinoacetate methyltransferase (GAMT) to
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