Immobilon^® Block - PO (Phosphoprotein Blocker)

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

이해합니다. 서양인들은 얼룩으로 인해 고통스러울 수 있으며, 저희도 확실히 그 고통을 겪었습니다. 그러니 여러분의 못생긴 오점을 공유해 주시고 깜짝 선물을 받아보세요! 깜짝 선물이 무엇인지 알려드릴 수는 없지만, 실험실에서 창의력을 자극할 수 있다는 점만 말씀드릴 수 있습니다.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.