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Merck

MAB5326

Sigma-Aldrich

Anti-Nestin Antibody, clone 10C2

clone 10C2, Chemicon®, from mouse

동의어(들):

Anti-Nestin

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

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기술 서비스
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기술 서비스
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도움 문의

생물학적 소스

mouse

Quality Level

100

결합

unconjugated

항체 형태

purified immunoglobulin

클론

10C2, monoclonal

종 반응성

human

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rat, mouse

포장

antibody small pack of 25 μg

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

입력

sample type epithelial cells
sample type neural stem cell(s)

동형

IgG1

NCBI 수납 번호

NM_006617.1

UniProt 수납 번호

P48681

배송 상태

ambient

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

200-220 kDa
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

면역원

Epitope: human
Fusion protein.

애플리케이션

Immunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein.

WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive.

Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton

Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells

Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells
This Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin.

생화학적/생리학적 작용

Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin.

물리적 형태

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

제조 메모

Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

분석 메모

Control
Positive Control: WI-38 (ATCC# CCL75) or U251 cell lines

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB5922

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Bone marrow-derived mesenchymal stem cells already express specific neural proteins before any differentiation.
Tondreau, Tatiana, et al.
Differentiation, 72, 319-326 (2004)
Transient expression of keratin during neuronal development in the adult rabbit spinal ganglion.
Iwatsuki H, Suda M
Anatomical Science International null
beta-cell neogenesis induced by adenovirus-mediated gene delivery of transcription factor pdx-1 into mouse pancreas.
Taniguchi, H, et al.
Gene Therapy, 10, 15-23 (2003)
Expression of stem-cell markers (cytokeratin 15 and nestin) in primary adnexal neoplasms-clues to etiopathogenesis.
Mahalingam M, Srivastava A, Hoang MP
The American Journal of Dermatopathology null
Multiple neurogenic and neurorescue effects of human mesenchymal stem cell after transplantation in an experimental model of Parkinson's disease.
Cova, Lidia, et al.
Brain Research, 1311, 12-27 (2010)
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