MAB5272
Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
clone 324, Chemicon®, from rat
동의어(들):
CD171, N-CAM L1
생물학적 소스
rat
Quality Level
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
324, monoclonal
종 반응성
mouse, rat
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... L1CAM(3897), VCAM1(7412)
일반 설명
120-140 kDa
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
면역원
Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
애플리케이션
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
생화학적/생리학적 작용
Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
물리적 형태
Format: Purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum.
Lindner, J, et al.
Nature, 305, 427-430 (1983)
Absence of layer-specific cadherin expression profiles in the neocortex of the reeler mutant mouse.
Hertel, N; Redies, C
Cerebral Cortex (1991)
Heparan sulfate sugar modifications mediate the functions of slits and other factors needed for mouse forebrain commissure development.
Conway, CD; Howe, KM; Nettleton, NK; Price, DJ; Mason, JO; Pratt, T
The Journal of Neuroscience null
Expression of ABCA2 protein in both non-myelin-forming and myelin-forming Schwann cells in the rodent peripheral nerve.
Takashi Saito, Katsuya Yamada, Yan Wang, Yukiko Tanaka, Kazuo Ohtomo, Kazuo Ishikawa, Nobuya Inagaki
Neuroscience Letters null
Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
Testing null
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