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Merck

MAB360

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

CHEMICON®, mouse monoclonal, GA5

동의어(들):

GFAP

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

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기술 서비스
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도움 문의
기술 서비스
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도움 문의

제품 이름

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5, ascites fluid, clone GA5, Chemicon®

생물학적 소스

mouse

Quality Level

100

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

GA5, monoclonal

종 반응성

rat, human, mouse, rabbit, chicken, pig, bovine

포장

antibody small pack of 25 μL

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

동형

IgG1

NCBI 수납 번호

NM_002055.2

UniProt 수납 번호

P14136

배송 상태

ambient

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... GFAP(2670)

관련 카테고리

일반 설명

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
~ 51 kDa

면역원

Purified GFAP from porcine spinal cord.

애플리케이션

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.

Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

Immunoblotting: A 1:1000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user

생화학적/생리학적 작용

Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.

물리적 형태

Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.

분석 메모

Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Replaces: 04-1031; 04-1062

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

O Nesic et al.
Neuroscience, 143(3), 779-792 (2006-11-01)
The effect of spinal cord injury (SCI) on the expression levels and distribution of water channel aquaporin 4 (AQP4) has not been studied. We have found AQP4 in gray and white matter astrocytes in both uninjured and injured rat spinal
Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons.
Das, M; Patil, S; Bhargava, N; Kang, JF; Riedel, LM; Seal, S; Hickman, JJ
Biomaterials null
Expression of leptin and leptin receptor isoforms in the rat and human carotid body.
Porzionato A, Rucinski M, Macchi V, Stecco C, Castagliuolo I, Malendowicz LK, De Caro R
Brain Research null
Cellular organization of adult neurogenesis in the Common Marmoset.
Bunk, Eva C, et al.
Aging Cell, 10, 28-38 (2011)
L-M Chen et al.
Neuroscience, 151(2), 374-385 (2007-12-07)
NCBE (SLC4A10) is a member of the SLC4 family of bicarbonate transporters, several of which play important roles in intracellular-pH regulation and transepithelial HCO(3)(-) transport. Here we characterize a new antibody that was generated in rabbit against a fusion protein
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프로토콜

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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