제품 이름
Anti-Vimentin Antibody, clone V9, clone V9, Chemicon®, from mouse
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
V9, monoclonal
종 반응성
monkey, feline, pig, bovine, rat, human, chicken, canine
반응하면 안 됨
mouse
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... VIM(7431)
일반 설명
57-60 kDa
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
면역원
Purified vimentin from pig eye lens
애플리케이션
Anti-Vimentin Antibody, clone V9 is an antibody against Vimentin for use in IC, IH, IH(P) & WB.
Immunoblotting:
A dilution of 1:1000 was used in a previous lot.
Immunohistochemistry:
5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunocytochemistry:
5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.
A dilution of 1:1000 was used in a previous lot.
Immunohistochemistry:
5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunocytochemistry:
5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.
생화학적/생리학적 작용
Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin
물리적 형태
Format: Purified
Purified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
분석 메모
Evaluated by Western Blot on HeLa lysates.
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3410
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Transplantation of multipotent cells extracted from adult skeletal muscles into the subventricular zone of adult rats.
Laurence Mignon,
The Journal of Comparative Neurology null
H J Gröne et al.
The American journal of pathology, 129(1), 1-8 (1987-10-01)
Most renal cell carcinomas coexpress vimentin and keratin, while renal tubular epithelia express only keratin. Investigation of the intermediate filament composition of tubular epithelia in diseased rat and human kidneys now shows that altered tubular epithelia unequivocally coexpress keratin and
Expression of amyloid precursor protein-like molecule in astroglial cells of the subventricular zone and rostral migratory stream of the adult rat forebrain.
Yasuoka, K; Hirata, K; Kuraoka, A; He, JW; Kawabuchi, M
Journal of Anatomy null
Zhan Ma et al.
Scientific reports, 2, 743-743 (2012-10-19)
Germline/embryonic-specific genes have been found to be activated in somatic tumors. In this study, we further showed that cells functioning as germline could be present in mouse fibrosarcoma cells (L929 cell line). Early germline-like cells spontaneously appeared in L929 cells
Edyta Kawka et al.
American journal of physiology. Renal physiology, 313(5), F1116-F1123 (2017-07-21)
Fibrotic thickening of the peritoneum develops in patients receiving peritoneal dialysis (PD) for renal failure. For unknown reasons, however, in some patients it progresses to extensive fibrosis that compromises dialysis capacity of the peritoneum. It is increasingly clear that fibroblasts
프로토콜
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
어떻게 오가노이드를 염색합니까? 항체를 이용한 오가노이드 배양에서 면역형광(IF) 및 면역세포화학(ICC) 염색에 대한 완전한 단계별 프로토콜
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.