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Merck

MAB318

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody

CHEMICON®, mouse monoclonal, LNC1

동의어(들):

TH, Tyrosine Monooxygenase, Tyrosine 3-monooxygenase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

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기술 서비스
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기술 서비스
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도움 문의

제품 이름

Anti-Tyrosine Hydroxylase Antibody, clone LNC1, ascites fluid, clone LNC1, Chemicon®

생물학적 소스

mouse

Quality Level

100

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

LNC1, monoclonal

종 반응성

zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken

제조업체/상표

Chemicon®

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG1κ

NCBI 수납 번호

NM_000360.3

UniProt 수납 번호

P07101

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... TH(7054)
rat ... Th(25085)

일반 설명

59-63 kDa
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

면역원

Tyrosine Hydroxylase purified from PC12 cells

애플리케이션

Detect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
Immunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).

Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

생화학적/생리학적 작용

Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).

물리적 형태

Ascites mouse monoclonal IgG1κ fluid containing PBS pH 7.1 , contains no preservative.
Unpurified

제조 메모

Stable for 1 year at -20ºC from date of receipt.

분석 메모

Control
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Routinely evaluated by Western Blot on Mouse Brain lysates.

Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation.
Anderson, GM; Beijer, P; Bang, AS; Fenwick, MA; Bunn, SJ; Grattan, DR
Endocrinology null
A FRET-based method to study protein thiol oxidation in histological preparations.
Mastroberardino, PG; Orr, AL; Hu, X; Na, HM; Greenamyre, JT
Free radical biology & medicine null
Expression patterns of the opsin 5-related genes in the developing chicken retina.
Sayuri Tomonari,Kyoichi Migita,Akira Takagi,Sumihare Noji,Hideyo Ohuchi
Developmental Dynamics null
Age and region-specific responses of microglia, but not astrocytes, suggest a role in selective vulnerability of dopamine neurons after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine exposure in monkeys.
Kanaan, NM; Kordower, JH; Collier, TJ
Glia null
Paraskevi Krashia et al.
Nature communications, 10(1), 4725-4725 (2019-10-16)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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