MAB3120
Anti-Cre Recombinase Antibody, clone 2D8
ascites fluid, clone 2D8, Chemicon®
동의어(들):
Anti-Cre Antibody
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
2D8, monoclonal
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG1κ
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
Escherichia coli ... Cre(2777477)
일반 설명
38 kDa
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. Anti-Cre Recombinase Antibody can be used to determine the success of gene manipulations in transgenic animals.
면역원
Cre recombinase (aa 77 to 343) fusion protein
애플리케이션
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.
Immunofluorescence:
A previous lot of this antibody was used on IF.
Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions must be determined by the end user.
생화학적/생리학적 작용
Cre recombinase. The epitope is located between aa 77 and 343.
물리적 형태
Ascites fluid. Contains no preservative
Unpurified
제조 메모
Stable for 1 year at -20ºC from date of receipt.
분석 메모
Control
HeLa Nuclear Extract
HeLa Nuclear Extract
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Murine cell lines derived from Pten null prostate cancer show the critical role of PTEN in hormone refractory prostate cancer development.
Jiao, J; Wang, S; Qiao, R; Vivanco, I; Watson, PA; Sawyers, CL; Wu, H
Cancer Research null
Hong-Xiang Liu et al.
Developmental biology, 368(2), 294-303 (2012-06-05)
The epithelium of mammalian tongue hosts most of the taste buds that transduce gustatory stimuli into neural signals. In the field of taste biology, taste bud cells have been described as arising from "local epithelium", in distinction from many other
Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration.
Judy Grover, Peter J Roughley
Matrix Biology null
Axon position within the corpus callosum determines contralateral cortical projection.
Zhou, J; Wen, Y; She, L; Sui, YN; Liu, L; Richards, LJ; Poo, MM
Proceedings of the National Academy of Sciences of the USA null
Quality control of astrocyte-directed Cre transgenic mice: the benefits of a direct link between loss of gene expression and reporter activation.
Robert Pascal Requardt, Lech Kaczmarczyk, Pavel Dublin, Anke Wallraff-Beck et al.
Glia null
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