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Merck

MAB1281

Sigma-Aldrich

Anti-Nuclei Antibody, clone 235-1

clone 235-1, Chemicon®, from mouse

동의어(들):

HuNu

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

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기술 서비스
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도움 문의
기술 서비스
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도움 문의

생물학적 소스

mouse

Quality Level

100

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

235-1, monoclonal

종 반응성

human

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

입력

sample type neural stem cell(s)

동형

IgG1

배송 상태

dry ice

타겟 번역 후 변형

unmodified

일반 설명

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Anti-nuclei antibodies are useful cytological and histological markers for identifying human cells in xenograft models.

애플리케이션

Anti-Nuclei Antibody, clone 235-1 has been used in: Immunohistochemistry (1:200); Immunolabeling for human nuclei (HuNu) (1:300); Histological and immuno-fluorescent analysis of submandibular glands

생화학적/생리학적 작용

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.

물리적 형태

Format: Purified
Hybridoma supernatant. Liquid in PBS. Contains no preservative.
Protein A purified

제조 메모

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
All human cell types

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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Long-term hibernation of human fetal striatal tissue does not adversely affect its differentiation in vitro or graft survival: implications for clinical trials in Huntington's disease.
Hurelbrink, Carrie B, et al.
Cell Transplantation, 12, 687-695 (2003)
Improving the survival of human CNS precursor-derived neurons after transplantation.
Le Belle, J E, et al.
Journal of Neuroscience Research, 76, 174-183 (2004)
Infusion of human umbilical cord blood cells in a rat model of stroke dose-dependently rescues behavioral deficits and reduces infarct volume.
Vendrame, Martina, et al.
Stroke, 35, 2390-2395 (2004)
Transplantation of cultured neural cells from human fetuses into the brain of rats exposed to acute hypoxia.
Aleksandrova, M A, et al.
Bulletin of experimental biology and medicine, 137, 262-265 (2004)
Human umbilical cord blood cells express neural antigens after transplantation into the developing rat brain.
Zigova, Tanja, et al.
Cell Transplantation, 11, 265-274 (2002)
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Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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