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Merck

ABT28

Sigma-Aldrich

Anti-Autotaxin Antibody

from rabbit, purified by affinity chromatography

동의어(들):

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

모든 문서 보기
기술 서비스
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도움 문의
기술 서비스
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도움 문의

생물학적 소스

rabbit

Quality Level

100

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human

종 반응성(상동성에 의해 예측)

canine (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)

기술

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI 수납 번호

NP_001035181

UniProt 수납 번호

Q13822

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... ENPP2(5168)

일반 설명

Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.
~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.

면역원

Epitope: Substrate binding region
KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.

애플리케이션

Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P).
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.

생화학적/생리학적 작용

This antibody recognizes Autotaxin at the substrate binding region.

분석 메모

Control
Oligodendrocyte precursor cell lysate
Evaluated by Western Blot in oligodendrocyte precursor cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Paul A Volden et al.
Cancer prevention research (Philadelphia, Pa.), 9(5), 367-378 (2016-02-11)
Lysophosphatidic acid (LPA), acting in an autocrine or paracrine fashion through G protein-coupled receptors, has been implicated in many physiologic and pathologic processes, including cancer. LPA is converted from lysophosphatidylcholine (LPC) by the secreted phospholipase autotaxin (ATX). Although various cell
Filipa L Cardoso et al.
Journal of neuroinflammation, 12, 82-82 (2015-05-01)
The inflammatory mediator lipopolysaccharide (LPS) has been shown to induce acute gliosis in neonatal mice. However, the progressive effects on the murine neurodevelopmental program over the week that follows systemic inflammation are not known. Thus, we investigated the effects of

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