AB9610

Anti-OLIG2 Antibody

Name: Anti-OLIG2 Antibody
Brand: MM

CHEMICON^®, rabbit polyclonal

동의어(들):

Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:

12352203

eCl@ss:

32160702

NACRES:

NA.41

주요 문서

모든 문서 보기

기술 서비스

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도움 문의

기술 서비스

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도움 문의

제품 이름

Anti-Olig-2 Antibody, Chemicon^®, from rabbit

생물학적 소스

rabbit

Quality Level

100

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

mouse, human, rat

제조업체/상표

Chemicon^®

기술

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI 수납 번호

NM_005806.2

UniProt 수납 번호

Q13516

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... OLIG2(10215)

일반 설명

Oligodendrocyte transcription factor 2 (UniProt: Q13516, also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is expressed in the brain in oligodendrocytes. Its strong expression is also observed in oligodendrogliomas. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It functions together with ZNF488 to promote oligodendrocyte differentiation. Olig2 is observed in both nucleus and the cytoplasm. Its nuclear localization signal is contained in the bHLH domain (aa 108-162). It can be masked in the native form and translocation to the nucleus may be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Olig2 cooperates with Olig1 to establish the motor neuron progenitor domain of the embryonic neural tube. Removal of Cdx transcription factors is shown to result in the continued induction of Olig2 and ectopic production of cranial motor neuron progenitors. A chromosomal aberration involving Olig2 is shown to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Metzis, V., et al. (2018). Cell 175(4); 1105-1118).

~32 kDa

면역원

Recombinant mouse Olig-2

애플리케이션

Anti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Western Blot Analysis:
1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

Immunoprecipitation:
1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
Optimal working dilutions must be determined by the end user.

Immunocytochemistry:
1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

Optimal working dilutions must be determined by the end user

생화학적/생리학적 작용

Other species have not been tested.

Recognizes the ~32 kDa Olig-2 protein by Western blot.

물리적 형태

Ammonium Sulfate Precipitation

Format: Purified

Purified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0.

제조 메모

Stable for 6 months at 2-8ºC from date of receipt.

분석 메모

Control
Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate

Evaluated by immunohistochemistry on glioblastoma

Immunohistochemistry (Paraffin):
Representative lot data.
Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB.
Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Brain abnormalities and glioma-like lesions in mice overexpressing the long isoform of PDGF-A in astrocytic cells.

Inga Nazarenko et al.

PloS one, 6(4), e18303-e18303 (2011-04-15)

Deregulation of platelet-derived growth factor (PDGF) signaling is a hallmark of malignant glioma. Two alternatively spliced PDGF-A mRNAs have been described, corresponding to a long (L) and a short (S) isoform of PDGF-A. In contrast to PDGF-A(S), the PDGF-A(L) isoform

Dorsally and ventrally derived oligodendrocytes have similar electrical properties but myelinate preferred tracts.

Tripathi, Richa B, et al.

The Journal of Neuroscience, 31, 6809-6819 (2011)

A novel transient glutamatergic population migrating from the pallial-subpallial boundary contributes to neocortical development.

Teissier, A; Griveau, A; Vigier, L; Piolot, T; Borello, U; Pierani, A

The Journal of Neuroscience null

Human neural progenitors from different foetal forebrain regions remyelinate the adult mouse spinal cord.

Buchet, D; Garcia, C; Deboux, C; Nait-Oumesmar, B; Baron-Van Evercooren, A

Brain null

The probable cell of origin of NF1- and PDGF-driven glioblastomas.

Hambardzumyan, D; Cheng, YK; Haeno, H; Holland, EC; Michor, F

Testing null

모든 관련된 서류 보기

문서

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Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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Antibody Generation | Monoclonal vs Polyclonal Antibodies

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모두 보기

프로토콜

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Anti-OLIG2 Antibody

CHEMICON®, rabbit polyclonal

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

생물학적 소스

Quality Level

항체 형태

항체 생산 유형

클론

종 반응성

제조업체/상표

기술

NCBI 수납 번호

UniProt 수납 번호

배송 상태

타겟 번역 후 변형

유전자 정보

설명

일반 설명

면역원

애플리케이션

생화학적/생리학적 작용

물리적 형태

제조 메모

분석 메모

기타 정보

법적 정보

면책조항

안전성 정보

Storage Class Code

WGK

Flash Point (°F)

Flash Point (°C)

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

관련 참고 논문

관련 참고 프로토콜

문서

프로토콜

관련 콘텐츠

기술 서비스

CHEMICON^®, rabbit polyclonal